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Pyrimethamine (PYR) and sulphadoxine have been used as a long acting antimalarial drug in Artemisinin Combination Therapy (ACT) of falciparum malaria. Resistance to pyrimethamine is primarily conferred by a point mutation at codon 108 (AGC ? AAC, S108N) of Plasmodium falciparum dihydrofolate reductase (Pfdhfr) gene. We have introduced and developed a novel application for rapid detection of pyrimethamine resistance in P. falciparum by using loop mediated isothermal amplification assay in field conditions. To implement this novel application many set of LAMP primers were designed and optimized finally one set (FIPM1) of LAMP primer were selected which specifically distinguish pyrimethamine resistant mutants of P. falciparum. With the optimal LAMP primers, the concentration of LAMP components were optimized and the reaction conditions were set as 60-66°C for 45 min. High sensitivity had been observed with 7 parasite/µl in 102 to 105 (

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